期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 100A, 期 5, 页码 1356-1367出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34075
关键词
PEG hydrogels; vascular cells; modulus; cell proliferation; gene expression
资金
- Nemours Foundation
- National Institutes of Health [COBRE P20-RR020173, INBRE P20-RR016472, COBRE P20-RR017716]
- National Science Foundation [DGE-0221651]
Regenerative medicine approaches offer attractive alternatives to standard vascular reconstruction; however, the biomaterials to be used must have optimal biochemical and mechanical properties. To evaluate the effects of biomaterial properties on vascular cells, heparinized poly(ethylene glycol) (PEG)-based hydrogels of three different moduli, 13.7, 5.2, and 0.3 kPa, containing fibronectin and growth factor were utilized to support the growth of three human vascular cell types. The cell types exhibited differences in attachment, proliferation, and gene expression profiles associated with the hydrogel modulus. Human vascular smooth muscle cells demonstrated preferential attachment on the highest-modulus hydrogel, adventitial fibroblasts demonstrated preferential growth on the highest-modulus hydrogel, and human umbilical vein endothelial cells demonstrated preferential growth on the lowest-modulus hydrogel investigated. Our studies suggest that the growth of multiple vascular cell types can be supported by PEG hydrogels and that different populations can be controlled by altering the mechanical properties of biomaterials. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.
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