期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 100A, 期 9, 页码 2412-2420出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.34171
关键词
bone regeneration; organ printing; osteoinductive hydrogel matrices; apatitic nanoparticles; biphasic phosphate microparticles
资金
- Netherlands Organization for Scientific Research (NWO) [017.001.181]
- Dutch Program for Tissue Engineering [UGT.6743]
- Dutch Technology Foundation STW (VENI) [08101]
- Anna Foundation
- Netherlands Ministry of Economic Affairs, The Netherlands Ministry of Education, Culture and Science
Hydrogels used as injectables or in organ printing often lack the appropriate stimuli to direct osteogenic differentiation of embedded multipotent stromal cells (MSCs), resulting in limited bone formation in these matrices. Addition of calcium phosphate (CaP) particles to the printing mixture is hypothesized to overcome this drawback. In this study we have investigated the effect of CaP particles on the osteoinductive potential of cell-laden hydrogel-CaP composite matrices. To this end, apatitic nanoparticles have been included in Matrigel constructs where after the viability of embedded progenitor cells was assessed in vitro. In addition, the osteoinductive potential of cell-laden Matrigel containing apatitic nanoparticles was investigated in vivo and compared with composites containing osteoinductive biphasic calcium phosphate (BCP) microparticles after subcutaneous implantation in immunodeficient mice. Histological and immunohistochemical analysis of the tissue response as well as in vivo bone formation revealed that apatitic nanoparticles were osteoinductive and induced osteoclast activation, but without bone formation. The BCP particles were more effective in inducing elaborate bone formation at the ectopic location. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 24122420, 2012
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