Processing-structure-functional property relationship in organic-inorganic nanostructured scaffolds for bone-tissue engineering: The response of preosteoblasts

We elucidate here for the first time the structureprocessingfunctional property relationship in chitosan (CS)-based scaffolds, where molecular machinery governing proliferation and growth of osteoblasts is mediated by nanostructured carbon. The interconnected network structure of organicinorganic scaffolds was obtained by covalent linkage of carboxyl group of functionalized single-walled carbon nanohorn with the amine group of CS. The molecular-scale dispersibility of functionalized nanostructured carbon was an important physicochemical factor influencing cellular interactions and biological response. Furthermore, it was beneficial in promoting the biocompatibility and the degradation product of the scaffolds. The hydrophilicity, good water retention ability, and interconnected porous structure of organicinorganic scaffolds enabled pronounced cell attachment and proliferation and enhanced the stability toward enzymatic degradation. The infiltration of cells and colonization of the pores of the scaffolds and cellular interactions were promoted due to covalent linkage of nanostructured carbon with CS. Additionally, the interconnectivity of porous scaffolds facilitated cells to infiltrate inside the pores of CS-nanostructured scaffolds, implying that nanostructured carbon merits consideration in tissue engineering. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:30803091, 2012.