Electrospun nanofiber-based regeneration of cartilage enhanced by mesenchymal stem cells

Application of biomaterials in combination with stem cells is a novel tissue engineering approach to regenerate cartilage. The objective of this study was to investigate the potential of poly(vinyl alcohol)/polycaprolactone (PVA/PCL) nanofiber scaffolds seeded with rabbit bone marrow-mesenchymal stem cell (BM-MSC) for cartilage tissue engineering in vitro and in vivo. We tested the biocompatibility and mechanical properties of nanofibrous scaffolds using scanning electron microscope, MTT assay, and tensile measurements. The capacity of MSC for chondrogenic differentiation on scaffolds was examined using reverse transcription-polymer chain reaction and immunostaining. For in vivo assessments, PVA/PCL nanofiber scaffolds with or without MSC were implanted into rabbit full-thickness cartilage defects. To evaluate cartilage regeneration, semi-quantitative grading and histological analysis were performed. Our results showed that PVA/PCL scaffolds supported the proliferation and chondrogenic differentiation of MSC in vitro. Moreover, the animals treated with cell-seeded PVA/PCL scaffolds showed improved healing of defects compared with untreated control and those which received cell-free scaffolds. Our findings suggest that PVA/PCL scaffolds incorporated with MSC can serve as a suitable graft for articular cartilage reconstruction. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 467-478, 2011.