期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 95A, 期 4, 页码 1233-1243出版社
WILEY
DOI: 10.1002/jbm.a.32940
关键词
transplantation; retinal pigment epithelium; poly(L-lactic acid); poly(D,L-lactic-co-glycolic acid); microcarriers
资金
- National Institute for Health Research
- National Eye Research Centre
- British Retinitis Pigmentosa Society
- Foresight RP
- National Institutes of Health Research (NIHR) [II-FS-0109-11028] Funding Source: National Institutes of Health Research (NIHR)
- National Institute for Health Research [II-FS-0109-11028, NF-SI-0507-10094] Funding Source: researchfish
Five blends of poly(L-lactic acid) (PLLA) with poly(D,L-lactic-co-glycolic acid) (PLGA) were used to prepare microcarriers by a solvent evaporation technique. Microcarriers were evaluated as suitable scaffolds to facilitate retinal pigment epithelium (RPE) cell transplantation. The blend ratios were 90:10, 75:25, 50:50, 25:75, 10:90 (PLLA:PLGA). Samples of each microcarrier blend were coated with an extracellular matrix protein. Coated and uncoated microcarriers were seeded with a human RPE cell line. As the lactide unit content increased the size of microcarriers generally became larger and the surface more irregular. Cells remained proliferative and retained phenotype as confirmed by immunocytochemistry. Blends rich in PLLA were superior for maintenance of RPE cell viability. This study demonstrates for the first time the feasibility of using microcarriers as a vehicle for retinal cell transplantation for ocular disease. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 1233-1243, 2010.
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