期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 95A, 期 1, 页码 283-293出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.32825
关键词
PEG hydrogel; fibrin ribbons; scaffold vascularization; coculture; endothelial cells
资金
- Beta Cell Biology Consortium [VUMC35144]
- National Institute of Diabetes and Digestive and Kidney Diseases [F30DK081278]
- Department of Education
Developing a mechanism to vascularize tissue-engineered constructs is imperative for transplant function and integration, particularly when delivering hypoxia-sensitive tissues, such as pancreatic islets. Previous efforts have focused on bulk modifications of scaffold materials rendering the entire construct permissive to vessel penetration or the formation of a porous structure where vessels can infiltrate the empty spaces. Here, we describe a novel construct composed of large fibrin ribbons encapsulated within a poly(ethylene glycol) (PEG) hydrogel. The PEG/fibrin ribbon composite scaffold facilitates coculture of adhesive and nonadhesive cell types, thus providing closely neighboring environments with distinct material properties specific to the needs of two clinically relevant cell populations. This advantage is demonstrated here by the successful coculture of pancreatic islets in the PEG component and vessel-forming endothelial cells in entrapped fibrin ribbons. Transplanted endothelial cells can form anastomosies with host vasculature, suggesting that our cocultures may lead to more rapid scaffold vascularization. Additionally, we show that surface-seeded endothelial cells form multicellular projections that migrate into nonadhesive PEG hydrogels along permissive fibrin ribbons, further demonstrating composite construct vascularization potential. Distribution of large fibrin ribbons throughout PEG hydrogels provide a potential mechanism for vascularization of a well-established biomaterial without inherently changing its desirable properties. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 283-293, 2010.
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