期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 93A, 期 2, 页码 673-686出版社
WILEY
DOI: 10.1002/jbm.a.32485
关键词
neuroblastoma; cell shape; extracellular matrix; micropattern; Pluronic
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [NIH5P60 DK20572]
- NIH [T32 NSO7222, K08EB03996]
- JDRF Center for the Study of Complications in Diabetes
- Program for Understanding Neurological Diseases
Influencing cell shape using micropatterned substrates affects cell behaviors, such as proliferation and apoptosis. Cell shape may also affect these behaviors in human neuroblastoma (NBL) cancer, but to date, no substrate design has effectively patterned multiple clinically important human NBL lines. In this study, we investigated whether Pluronic F108 was an effective antiadhesive coating for human NBL cells and whether it would localize three NBL lines to adhesive regions of tissue culture plastic or collagen I on substrate patterns. The adhesion and patterning of an S-type line, SH-EP, and two N-type lines, SH-SY5Y and IMR-32, were tested. In adhesion assays, F108 deterred NBL adhesion equally as well as two antiadhesive organofunctional silanes and far better than bovine serum albumin. Patterned stripes of F108 restricted all three human NBL lines to adhesive stripes of tissue culture plastic. We then investigated four schemes of applying collagen and F108 to different regions of a substrate. Contact with collagen obliterates the ability of 1:108 to deter NBL adhesion, limiting how both materials can be applied to substrates to produce high fidelity NBL patterning. This patterned substrate design should facilitate investigations of the role of cell shape in NBL cell behavior. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 673-686, 2010
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据