期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 92A, 期 3, 页码 988-996出版社
WILEY
DOI: 10.1002/jbm.a.32341
关键词
cartilage tissue engineering; p(NiPAAm-co-AAc); human mesenchymal stem cells; thermosensitive hydrogel; transforming growth factor-beta 3 (TGF-beta 3)
资金
- Gyeonggi Regional Research Center (GRRC), Catholic University of Korea
- National Research Foundation of Korea [핵09A3504] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this Study, hi vivo studies, both nude Mouse and rabbit cartilage defect, were tested for chondrogenesis using stem cells (SCS) using growth factor. Specifically, human mesenchymal stem cells (hMSCs) were embedded in a hydrogel scaffold, which was coencapsulated with transforming growth factor-beta 3 (TGF-beta 3). The specific extracellular matrices (ECMs) released from hMSCs transplanted into the animal were assessed via glycosaminoglycan (GAG)/DNA content, RT-PCR, real time-QPCR, immunohistochemical (IHC), and Safranin-O staining and were observed Lip to 7 weeks after injection. By detection of ECMs the GAG content per cell remained constant for all formulations, indicating that the dramatic increase ill cell number for samples with TGF-beta 3 was accompanied by the maintenance of the cell phenotypes. The histological and IHC staining of the newly repaired tissues observed after treatment with TGF-beta 3 mixed with hMSCs evidenced hyvaline cartilage-like characteristics. Moreover, the results Observed with the animal model (rabbit) treated with hMSCs embedded in the growth factor-containing hydrogel indicate that the implantation of mixed cells with TGF-beta 3 may constitute a clinically efficient method for the,generation of hyaline articular cartilage. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A; 988-996, 2010
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