期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 87A, 期 4, 页码 1075-1085出版社
WILEY
DOI: 10.1002/jbm.a.31851
关键词
acellularization; decellularization; embryonic stem cells; embryoid bodies; extracellular matrix
资金
- Georgia Tech/Emory Center for the Engineering of Living Tissues (GTEC) [NSF EEC-9731463]
- American Heart Association [0665265B]
Embryonic stem cells (ESCs) can differentiate into all somatic cell types, thereby providing a robust cell Source for regenerative medicine therapies. ESCs are commonly induced to differentiate via three-dimensional cell aggregates called embryoid bodies (EBs), which recapitulate cellular and molecular aspects of early tissue morphogenesis. Recent studies suggest that biomolecules synthesized by transplanted ESCs may provide instructive cues for tissue regeneration in vivo. Thus the objective of this study was to acellularize EBs at different stages of differentiation in order to extract extracellular matrices containing ESC-derived biomolecules. Successive treatment with Triton X-100 and DNase significantly reduced the cellularity and completely inhibited the viability of EBs at various stages of differentiation. In addition, most DNA content (69-75%,) was removed, while a portion of the original protein content (15-25%,) was retained. Most importantly, extracellular matrix components produced by EBs were retained after acellularization. These results indicate that Successful acellularization of EBs can be performed at various stages of differentiation to enable temporal modulation of acellular ECM composition. In addition, acellular matrices derived from EBs represent a novel route of obtaining molecular Cues produced by ESCs actively undergoing morphogenesis, thus this technology be relevant evant to the development Of future, regenerative medicine therapies. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 1075-1085, 2008
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据