期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 85A, 期 2, 页码 313-325出版社
WILEY-LISS
DOI: 10.1002/jbm.a.31379
关键词
microgroove; in vitro; cell-surface interactions; fibroblasts; bone marrow
Surface microgeometry plays a role in tissue-implant surface interactions, but our understanding of its effects is incomplete. Substrate microgrooves strongly influence cells in vitro, as evidenced by contact guidance and cell alignment. We studied dot colonies of primary fibroblasts and bone marrow cells that were grown on titanium-coated, microgrooved polystyrene surfaces that we designed and produced. Rat tendon fibroblast and rat bone marrow colony growth and migration varied (p < 0.01) by microgroove dimension and slightly by cell type. We observed profoundly altered morphologies, reduced growth rates, and directional growth in colonies grown on microgrooved substrates, when compared with colonies grown on flat, control surfaces (p < 0.01). The cells in our colonies grown on microgrooved surfaces were well aligned and elongated in the direction parallel to the grooves and colonies. Our dot colony is an easily reproduced, easily measured and artificial explant model of tissue-implant interactions that better approximates in vivo implant responses than culturing isolated cells on biomaterials. Our results correlate well with in vivo studies of titanium dioxide-coated polystyrene, titanium, and titanium alloy implants with controlled microgeometries. Microgrooves and other surface features appear to directionally or spatially organize cells and matrix molecules in ways that contribute to improved stabilization and osseointegration of implants. (C) 2007 Wiley Periodicals, Inc.
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