期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 85A, 期 2, 页码 530-538出版社
WILEY-BLACKWELL
DOI: 10.1002/jbm.a.31281
关键词
cell microenvironment; parylene; patterning
资金
- NHLBI NIH HHS [R01 HL060435, HL60435] Funding Source: Medline
- NIDCR NIH HHS [R01 DE013023, R01 DE013023-08, DE16516, R01 DE016516] Funding Source: Medline
- Div Of Engineering Education and Centers
- Directorate For Engineering [832785] Funding Source: National Science Foundation
The patterned deposition of cells and biomolecules on surfaces is a potentially useful tool for in vitro diagnostics, high-throughput screening, and tissue engineering. Here, we describe an inexpensive and potentially widely applicable micropatterning technique that uses reversible seating of microfabricated parylene-C stencils on surfaces to enable surface patterning. Using these stencils it is possible to generate micropatterns and copatterns of proteins and cells, including NIH-3T3 fibroblasts, hepatocytes and embryonic stem cells. After patterning, the stencils can be removed from the surface, plasma treated to remove adsorbed proteins, and reused. A variety of hydrophobic surfaces including PDMS, polystyrene and acrylated glass were patterned using this approach. Furthermore, we demonstrated the reusability and mechanical integrity of the parylene membrane for at least 10 consecutive patterning processes. These parylene-C stencils are potentially scalable commercially and easily accessible for many biological and biomedical applications. (C) 2007 Wiley Periodicals, Inc.
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