期刊
JOURNAL OF BIOMECHANICS
卷 43, 期 4, 页码 783-787出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2009.10.022
关键词
Articular cartilage; Chondrocytes; Osmotic loading; Finite element analysis; Fibril-reinforced
资金
- Alberta Heritage Foundation for Medical Research, Alberta, Canada
- Canadian Institutes of Health Research
- Canada Research Chair Programme
- Alberta Ingenuity Fund, Canada
- Academy of Finland [125415]
- Sigrid Juselius Foundation
- Academy of Finland (AKA) [125415, 125415] Funding Source: Academy of Finland (AKA)
Osmotic loading is known to modulate chondrocyte (cell) height, width and volume in articular cartilage. It is not known how cartilage architecture, especially the collagen fibril orientation, affects cell shape changes as a result of an osmotic challenge. Intact patellae of New Zealand white rabbits (n = 6) were prepared for fluorescence imaging. Patellae were exposed to a hypotonic osmotic shock and cells were imaged before loading and 5-60 nun after the osmotic challenge. Cell volumes and aspect ratios (height/width) were analyzed. A fibril-reinforced poroelastic swelling model with realistic primary collagen fibril orientations, i.e. horizontal, random and vertical orientation in the superficial, middle and deep zones, respectively and cells in different zones was used to estimate cell aspect ratios theoretically. As the medium osmolarity was reduced, cell aspect ratios decreased and volumes increased in the superficial zone of cartilage both experimentally (p < 0.05) and theoretically. Theoretically determined aspect ratios of middle zone cells remained virtually constant, while they increased for deep zone cells as osmolarity was reduced. Findings of this study suggest that osmotic loading modulates chondrocyte shapes in accordance with the primary collagen fibril directions in articular cartilage. (C) 2009 Elsevier Ltd. All rights reserved.
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