期刊
JOURNAL OF BIOMECHANICS
卷 42, 期 10, 页码 1483-1487出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2009.03.050
关键词
Double-stranded DNA; Mechanical property; Axial compression; Young's modulus; Microcantilever
资金
- Natural Science Foundation of Shanghai Municipality [07ZR14037]
- Multiscale Material Mechanics Fellowship
- Research Innovation Program of Shanghai Education Commission [09YZ07]
- Natural Science Foundation of China [10872121]
- Graduate Student Innovation Foundation of Shanghai University [SHUCX080223]
- Shanghai Leading Academic Discipline Project [S30106]
- National Outstanding Young Scientists Foundation of China [10725209]
In label-free biodetections based on microcantilever technology, double-stranded DNA (dsDNA) structures form through the linkage between probe single-stranded DNA (ssDNA) molecules immobilized on solid substrates and target ssDNA molecules in solutions. Mechanical/electrical properties of these biolayers are important factors for nanomechanical deflections of microcantilevers. In this paper, the biolayer immobilized on microcantilever is treated as a bar with a macroscopic elastic modulus on the basis of continuum mechanics viewpoints. In consideration of hydration force, screened electrostatic repulsion and conformational fluctuation in biolayers, load-deformation curves of dsDNA biolayers under axial compression are depicted with the help of the energy conservation law and a mesoscopic free energy presented by Strey et al. (1997,1999) [Strey, H.H., Parsegian, V.A., Podgornik, R., 1997. Equation of state for DNA liquid crystals: fluctuation enhanced electrostatic double layer repulsion. Physical Review Letters 78, 895-898: Strey, H.H., Parsegian, V.A.. Podgornik, R., 1999. Equation of state for polymer liquid crystals: theory and experiment. Physical Review E 59, 999-1008] from a liquid crystal theory. And the analytical relation between macroscopic Young's modulus of biolayers and nanoscopic geometrical properties of dsDNA, packing density, buffer salt solution concentration, etc. is also formulated. (C) 2009 Elsevier Ltd. All rights reserved.
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