期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 24, 期 11, 页码 1368-1377出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2012.761058
关键词
bacterial nanocellulose; neuronal cells; polypyrrole; 3D cell culture; nanofibrous scaffolds
资金
- Brazilian Council for Scientific and Technological Development (CNPq)
- CAPES
- Portuguese Foundation for Science and Technology [SFRH/BPD/64958/2009]
In this work, polypyrrole (PPy) was in situ polymerized onto the surface of bacterial nanocellulose (BNC) produced by Gluconacetobacter xylinus, by chemical oxidation in aqueous medium using ammonium persulfate. Composites (BNC/PPy) were produced with varying concentrations of pyrrole (Py). The produced BNC/PPy membranes were used as a template for the seeding of PC12 rat neuronal cells. Cell suspensions were directly seeded onto the surfaces of the BNC/PPy membranes. The Py concentration affected the behavior of neuronal cells that adhered and grew significantly more on BNC/PPy comparatively to BNC. Scanning electron microscopy (SEM) micrographs revealed that PC12 cells adhered on the surface of the BNC and BNC/PPy membranes. Conductive PPy coatings on nanofibers acting as an active interface for tissue engineering may be used to regulate cell activity through electrical stimulations.
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