期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 24, 期 10, 页码 1233-1243出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2012.745717
关键词
osteogenic differentiation; adipose tissue-derived mesenchymal stem cells (Ad-MSCs); collagen I gel; serum-derived albumin scaffold
资金
- National Research Foundation of Korea (NRF)
- Korean government (MEST) [2009-0077045]
- Korea Research Foundation
- Korean Government [KRF-2010-0025387]
- National Research Foundation of Korea [2009-0077045, 2010-0025387] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Repair of bone defects is a difficult clinical problem for reconstructive surgeons. Bone tissue engineering using an appropriate scaffold with cells is a new therapy for the repair of bone defects. The aim of this study was to evaluate the in vitro osteogenesis of canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) cultured in a combination of collagen I gel and a porous serum-derived albumin scaffold. A serum-derived albumin scaffold was prepared with canine serum by cross-linking and freeze-drying procedures. Ad-MSCs were seeded into serum-derived albumin scaffolds with or without collagen I gel, and were exposed to osteogenic differentiation conditions in vitro. After 28days of in vitro culture, the distribution and osteogenic differentiation of Ad-MSCs cultured in the scaffold were evaluated by scanning electron microscopy, histology, immunohistochemistry, alkaline phosphatase (ALP) activity assay, and calcium colorimetric assay. Ad-MSCs showed more homogeneous distribution and osteogenic differentiation in the scaffold with collagen I gel than without collagen I gel. ALP activity and extracellular matrix mineralization in the construct with type I collagen were significantly higher than in the construct without type I collagen (p<0.05). In conclusion, the combination of collagen I gel and the serum-derived albumin scaffold enhanced osteogenic differentiation and homogenous distribution of Ad-MSCs.
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