期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 21, 期 3, 页码 359-376出版社
TAYLOR & FRANCIS LTD
DOI: 10.1163/156856209X416980
关键词
Ocular biocompatibility; hyaluronic acid; chemical cross-linking; hydrogel carrier; cell sheet delivery
资金
- National Science Council of Republic of China [NSC97-2221-E-182-003]
- Chang Gung Memorial Hospital [CMRPD170341]
- National Tsing Hua University [NTHU97N2551E1]
Due to its innocuous nature, hyaluronic acid (HA) is one of the most commonly used biopolymers for ophthalmic applications. We recently developed a cell sheet delivery system using carbodiimide crosslinked HA carriers. Chemical cross-linking provides an improvement in stability of polymer gels, but probably causes toxic side-effects. The aim of this study was to investigate the ocular biocompatibility of HA hydrogels cross-linked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC). HA discs without cross-linking and glutaraldehyde (GTA) cross-linked HA samples were used for comparison. The disc implants were inserted in the anterior chamber of rabbit eyes for 24 weeks and characterized by slit-lamp biomicroscopy, histology and scanning electron microscopy. The ophthalmic parameters obtained from biomicroscopic examinations were also scored to provide a quantitative grading system. Results of this study showed that the HA discs cross-linked with EDC had better ocular biocompatibility than those with GTA. The continued residence of GTA cross-linked HA implants in the intraocular cavity elicited severe tissue responses and significant foreign body reactions, whereas no adverse inflammatory reaction was observed after contact with non-cross-linked HA or EDC cross-linked HA samples. It is concluded that the cross-linking agent type gives influence on ocular biocompatibility of cell carriers and the EDC-HA hydrogel is an ideal candidate for use as an implantable material in cell sheet delivery applications. (C) Koninklijke Brill NV, Leiden, 2010
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