期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 21, 期 12, 页码 1637-1652出版社
TAYLOR & FRANCIS LTD
DOI: 10.1163/092050609X12525750021270
关键词
3-D porous scaffold; waterborne polyurethane; HUVEC; pore diameter
资金
- National 863project [2008AA03Z304]
- National Natural Science Foundation of China [20774061]
- Application of Basic Research Foundation of Sichuan [JH20075291539101]
In this study, a series of 3-D interconnected porous scaffolds with various pore diameters and porosities was fabricated by freeze-drying with non-toxic biodegradable waterborne polyurethane (WBPU) emulsions of different concentration. The structures of these porous scaffolds were characterized by scanning electron microscopy (SEM), and the pore diameters were calculated using CIAS 3.0 software. The pores obtained were 3-D interconnected in the scaffolds. The scaffolds obtained at different pre-freeze temperatures showed a pore diameter ranging from 2.8 to 99.9 mu m with a pre-freezing temperature of -60 degrees C and from 13.1 to 229.1 mu m with a pre-freezing temperature of -25 degrees C. The scaffolds fabricated with WBPU emulsions of different concentration at the same pre-freezing temperature (-25 degrees C) had pores with mean pore diameter between 90.8 and 39.6 mu m and porosity between 92.0 and 80.0%, depending on the emulsion concentration. The effect of porous structure of the scaffolds on adhesion and proliferation of human umbilical vein endothelial cells (HUVECs) cultured in vitro was evaluated using the MTT assay and environmental scanning electron microscopy (ESEM). It was found that the better adhesion and proliferation of HUVECs on 3-D scaffolds of WBPU with relative smaller pore diameter and lower porosity than those on scaffolds with larger pore and higher porosity and film. Our work suggests that fabricating a scaffold with controllable pore diameter and porosity could be a good method to be used in tissue-engineering applications to obtain carriers for cell culture in vitro. (C) Koninklijke Brill NV, Leiden, 2010
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据