期刊
JOURNAL OF BIOMATERIALS APPLICATIONS
卷 33, 期 2, 页码 216-226出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328218788242
关键词
Breast cancer; gene therapy; GO-HAp; nanoparticles; HSV-TK
Gene therapy with herpes simplex virus thymidine kinase gene (HSV-TK), which is also known as suicide gene therapy, is effective in various tumor models. The lack of a safe and efficient gene delivery system has become a major obstacle to suicide gene therapy. In this study, the cytotoxicity and transfection efficiency of graphene oxide-hydroxyapatite (GO-Hap) were analyzed by MTS and flow cytometry, respectively. A series of assays were performed to evaluate the effects of GO-HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment on growth of human breast normal and cancer cells. The results showed that GO-HAp nanocomposites effectively transfected cells with minimum toxicity. GO-HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment inhibited the proliferation and induced cell apoptosis in cancer cells, while the cytotoxic effects are tolerable in normal breast cells. We conclude that the GO-HAp nanocomposites have significant potential as a gene delivery vector for cancer therapy.
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