期刊
JOURNAL OF BIOMATERIALS APPLICATIONS
卷 28, 期 9, 页码 1354-1365出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328213508328
关键词
stability; biomaterial; Collagen; cell adhesion; biomimetic; recombinant protein; atomic force microscopy
资金
- National Science Foundation [CBET-1034566, DMR-1006999, CHE-0748912]
- Direct For Mathematical & Physical Scien
- Division Of Materials Research [1006999] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1034566] Funding Source: National Science Foundation
The ability to engineer bioactive sites within the biopolymer collagen has significant potential to dictate cellular microenvironments and processes. We have developed a novel recombinant DNA platform that enables such molecular-level control over this important material. In this investigation, we demonstrated the production of synthetic human collagen using yeast strains that were engineered with human prolyl hydroxylase alpha and beta genes integrated into the genome and a codon-optimized collagen gene carried on a plasmid. To understand the extent to which this synthetic collagen can mimic native human collagen, we examined the relationships between the structural topology and physical stability with the ability to support adhesion of HT-1080 cells. Characterization of these biopolymers included evaluation using circular dichroism spectroscopy, atomic force microscopy, and MTT metabolic activity assays. Although the apparent melting temperatures of the recombinant collagens were similar to 3-5celcius less than native sources, the recombinant and native collagens exhibited comparable triple helical structure, polymeric dimensions, adsorption on polystyrene, and cellular adhesion properties below their respective melting temperature values. These results support the feasibility of producing molecularly-engineered collagens that can mimic native substrates for therapeutic and tissue engineering applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据