期刊
JOURNAL OF BIOMATERIALS APPLICATIONS
卷 28, 期 6, 页码 922-936出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328213486705
关键词
Human dental pulp stem cells; differentiation; biomaterial; poly(lactide-co--caprolactone); collagen; hydroxyapatite; bone regeneration
资金
- Canadian Institutes of Health Research (CIHR)
- Natural Science and Engineering Research Council of Canada (NSERC)
- Reseau de recherche en sante buccodentaire et osseuse du FRSQ
The aim of this study was to design a new natural/synthetic bioactive bone scaffold for potential use in bone replacement applications. We developed a tri-component osteogenic composite scaffold made of collagen (Coll), hydroxyapatite (HA) and poly(l-lactide-co--caprolactone) (PLCL). This Coll/HA/PLCL composite scaffold was combined with human osteoblast-like cells obtained by differentiation of dental pulp stem cells (DPSCs) to engineer bone tissue invitro. Results show that the 3D Coll/HA/PLCL composite scaffold was highly porous, thereby enabling osteoblast-like cell adhesion and growth. Cultured in the Coll/HA/PLCL scaffold, the osteoblast-like cells expressed different osteogenic genes, produced alkaline phosphatase and formed nodules more than did PLCL alone. Micro-CT analyses revealed a significant (30%) increase of tissue mineralisation on the surface as well as inside of the Coll/HA/PLCL scaffold, thus confirming its effectiveness as a bone regeneration platform.
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