Biosynthesis of Calcium Hydroxylapatite Coating on Sputtered Ti/TiN Nano Multilayers and their Corrosion Behavior in Simulated Body Solution

Titanium/titanium nitride (Ti/TiN) nanoscale multilayered films were deposited onto 316L stainless steel substrates by reactive magnetron sputtering using a Ti target. Coatings characterized by X-ray diffraction showed that the stack possesses centered cubic structure. The X-ray photoelectron spectroscopy survey spectra on the etched surfaces of the stack film on steel exhibited the characteristic Ti2p, N1s, and O1s peaks at the corresponding binding energies 454.5, 397.0, and 530.6 eV, respectively. Platelet adhesion experiments were carried out to examine the interaction between blood and the materials in vitro. The results indicated that the smoothness and lower isoelectric point contribute to better hemocompatibility of the Ti/TiN nanoscale multilayered coating. The biomediated synthesis of calcium hydroxylapatite (HA) was carried out on coated substrates using calcium-depositing bacteria. The observation of low corrosion current density (I-corr) for the calcium HA-coated Ti/TiN specimens in simulated body fluid confirmed their highly resistive nature under the testing condition.