期刊
JOURNAL OF BIOLOGICAL PHYSICS
卷 34, 期 5, 页码 487-493出版社
SPRINGER
DOI: 10.1007/s10867-008-9107-y
关键词
Triple-color FRET (triFRET); Light-emitting polymer; Fluorescent dye; UV-visible absorption; Photoluminescence
类别
资金
- Department of Biotechnology (DBT), India [BT/PR6440/MED/14/812/2005]
Three-component Forster resonance energy transfer (FRET) has been used to obtain efficient FRET between the cationic conjugated polymer (CCP) as donor and 5-carboxy tetramethylrhodamine (TAMRA) dye as acceptor, by using an intermediate donor, fluorescein. In spite of the fact that there is enough overlap between the emission spectra of CCP and absorption spectra of TAMRA. the efficiency of FRET between CCP and TAMRA is poor. The reason for this is that while the Forster critical distance is not very sensitive to the overlap, the FRET efficiency is extremely sensitive to it. However, it is observed that the FRET efficiency between CCP and TAMRA improves considerably when fluorescein is introduced in the solution. The triple FRET so obtained can be used for deoxyribonucleic acid sequence detection in medical diagnostics because the fluorescence emission from TAMRA is pH-insensitive.
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