期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 9, 页码 5565-5579出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.511220
关键词
Directed Evolution; Immunology; Protein Folding; Structural Biology; von Willebrand Factor; Glycoprotein Ib; Yeast Display
资金
- National Institutes of Health [HL-103526, NIH-1F32HL-099167]
- American Heart Association [AHA-10POST4170043]
Background: Force in fluid flow regulates von Willebrand factor (VWF) A1 domain binding to glycoprotein Ib (GPIb). Results: X-ray crystal structures of high affinity A1-GPIb complexes and mutations reveal interactions involving central leucine-rich repeats of GPIb. Conclusion: Structural changes are on a pathway to a force-induced super high affinity state. Significance: A1-GPIb complexes provide insight into mechanochemistry of bleeding disorders. Activation by elongational flow of von Willebrand factor (VWF) is critical for primary hemostasis. Mutations causing type 2B von Willebrand disease (VWD), platelet-type VWD (PT-VWD), and tensile force each increase affinity of the VWF A1 domain and platelet glycoprotein Ib (GPIb) for one another; however, the structural basis for these observations remains elusive. Directed evolution was used to discover a further gain-of-function mutation in A1 that shifts the long range disulfide bond by one residue. We solved multiple crystal structures of this mutant A1 and A1 containing two VWD mutations complexed with GPIb containing two PT-VWD mutations. We observed a gained interaction between A1 and the central leucine-rich repeats (LRRs) of GPIb, previously shown to be important at high shear stress, and verified its importance mutationally. These findings suggest that structural changes, including central GPIb LRR-A1 contact, contribute to VWF affinity regulation. Among the mutant complexes, variation in contacts and poor complementarity between the GPIb -finger and the region of A1 harboring VWD mutations lead us to hypothesize that the structures are on a pathway to, but have not yet reached, a force-induced super high affinity state.
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