期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 10, 页码 6669-6680出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.520189
关键词
Amyotrophic Lateral Sclerosis (Lou Gehrig's Disease); Analytical Ultracentrifugation; Flow Cytometry; Polyglutamine; Protein Aggregation; Superoxide Dismutase (SOD); Inclusion; Polyalanine
资金
- Australian Research Council (ARC) Discovery Grant [DP120102763]
- ARC Future Fellowship Grant [FT120100039, FT100100560]
- Australian Research Council [FT120100039] Funding Source: Australian Research Council
Background: Protein aggregation is associated with neurodegenerative diseases. Results: We defined how the oligomeric state of disease-relevant mutant protein and homopolypeptides relate to clustering into inclusion subtypes IPOD and JUNQ. Conclusion: JUNQ protein and homopolypeptides relate to constitutively disrupted oligomeric states irrespective of inclusion formation. Significance: JUNQ inclusions may arise by cellular failure in degradation of abnormal oligomeric states. Protein aggregation into intracellular inclusions is a key feature of many neurodegenerative disorders. A common theme has emerged that inappropriate self-aggregation of misfolded or mutant polypeptide sequences is detrimental to cell health. Yet protein quality control mechanisms may also deliberately cluster them together into distinct inclusion subtypes, including the insoluble protein deposit (IPOD) and the juxtanuclear quality control (JUNQ). Here we investigated how the intrinsic oligomeric state of three model systems of disease-relevant mutant protein and peptide sequences relates to the IPOD and JUNQ patterns of aggregation using sedimentation velocity analysis. Two of the models (polyalanine (37A) and superoxide dismutase 1 (SOD1) mutants A4V and G85R) accumulated into the same JUNQ-like inclusion whereas the other, polyglutamine (72Q), formed spatially distinct IPOD-like inclusions. Using flow cytometry pulse shape analysis (PulSA) to separate cells with inclusions from those without revealed the SOD1 mutants and 37A to have abruptly altered oligomeric states with respect to the nonaggregating forms, regardless of whether cells had inclusions or not, whereas 72Q was almost exclusively monomeric until inclusions formed. We propose that mutations leading to JUNQ inclusions induce a constitutively misfolded state exposing hydrophobic side chains that attract and ultimately overextend protein quality capacity, which leads to aggregation into JUNQ inclusions. Poly(Q) is not misfolded in this same sense due to universal polar side chains, but is highly prone to forming amyloid fibrils that we propose invoke a different engagement mechanism with quality control.
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