期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 289, 期 33, 页码 23189-23199出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M114.567107
关键词
-
资金
- Department of Physiology and Biophysics, Dalhousie University
- Dalhousie Medical Research Foundation (DMRF)
- DMRF
- Canadian Institutes of Health Research (CIHR) [MOP-119349]
- CIHR [201109MSH-261462-208625]
- Nova Scotia Health Research Foundation [MED-PRO-2011-7485]
- Canada Foundation for Innovation Leaders Opportunity Fund [29291]
Lysosomes contain abundant ATP, which is released through lysosomal exocytosis following exposure to various stimuli. However, the molecular mechanisms underlying lysosomal ATP accumulation remain unknown. The vesicular nucleotide transporter, also known as solute carrier family 17 member 9 (SLC17A9), has been shown to function in ATP transport across secretory vesicles/granules membrane in adrenal chromaffin cells, T cells, and pancreatic cells. Here, using mammalian cell lines, we report that SLC17A9 is highly enriched in lysosomes and functions as an ATP transporter in those organelles. SLC17A9 deficiency reduced lysosome ATP accumulation and compromised lysosome function, resulting in cell death. Our data suggest that SLC17A9 activity mediates lysosomal ATP accumulation and plays an important role in lysosomal physiology and cell viability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据