期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 288, 期 45, 页码 32574-32584出版社
ELSEVIER
DOI: 10.1074/jbc.M113.503516
关键词
Bacterial Signal Transduction; Escherichia coli; Oxidative Stress; Redox Signaling; Transcription Regulation; Hypochlorous Acid (HOCl); Reactive Chlorine Species (RCS); YkgD
资金
- National Institutes of Health [R01-GM065318, R21-AI097893]
- National Research Service Award [F32GM096613]
Reactive chlorine species (RCS) such as hypochlorous acid are powerful antimicrobial oxidants. Used extensively for disinfection in household and industrial settings (i.e. as bleach), RCS are also naturally generated in high quantities during the innate immune response. Bacterial responses to RCS are complex and differ substantially from the well characterized responses to other physiologically relevant oxidants, like peroxide or superoxide. Several RCS-sensitive transcription factors have been identified in bacteria, but most of them respond to multiple stressors whose damaging effects overlap with those of RCS, including reactive oxygen species and electrophiles. We have now used in vivo genetic and in vitro biochemical methods to identify and demonstrate that Escherichia coli RclR (formerly YkgD) is a redox-regulated transcriptional activator of the AraC family, whose highly conserved cysteine residues are specifically sensitive to oxidation by RCS. Oxidation of these cysteines leads to strong, highly specific activation of expression of genes required for survival of RCS stress. These results demonstrate the existence of a widely conserved bacterial regulon devoted specifically to RCS resistance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据