期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 288, 期 47, 页码 34098-34109出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M113.516880
关键词
ABC Transporter; Actin; AMP-activated kinase (AMPK); Endocytosis; Potassium Channels; cAMP-dependent Protein Kinase (PKA); Signal Transduction
资金
- National Institutes of Health [DK57699, T32 DK007680]
Background: Leptin inhibits insulin secretion by increasing -cell K-ATP currents. Results: Leptin causes a transient increase in surface K-ATP channel density. This increase is dependent on AMPK and PKA and actin depolymerization. Conclusion: Leptin increases K-ATP currents by recruiting K-ATP channels to the -cell membrane. Significance: K-ATP channel trafficking is an important physiological mechanism to regulate channel activity. Pancreatic -cells secrete insulin in response to metabolic and hormonal signals to maintain glucose homeostasis. Insulin secretion is under the control of ATP-sensitive potassium (K-ATP) channels that play key roles in setting -cell membrane potential. Leptin, a hormone secreted by adipocytes, inhibits insulin secretion by increasing K-ATP channel conductance in -cells. We investigated the mechanism by which leptin increases K-ATP channel conductance. We show that leptin causes a transient increase in surface expression of K-ATP channels without affecting channel gating properties. This increase results primarily from increased channel trafficking to the plasma membrane rather than reduced endocytosis of surface channels. The effect of leptin on K-ATP channels is dependent on the protein kinases AMP-activated protein kinase (AMPK) and PKA. Activation of AMPK or PKA mimics and inhibition of AMPK or PKA abrogates the effect of leptin. Leptin activates AMPK directly by increasing AMPK phosphorylation at threonine 172. Activation of PKA leads to increased channel surface expression even in the presence of AMPK inhibitors, suggesting AMPK lies upstream of PKA in the leptin signaling pathway. Leptin signaling also leads to F-actin depolymerization. Stabilization of F-actin pharmacologically occludes, whereas destabilization of F-actin simulates, the effect of leptin on K-ATP channel trafficking, indicating that leptin-induced actin reorganization underlies enhanced channel trafficking to the plasma membrane. Our study uncovers the signaling and cellular mechanism by which leptin regulates K-ATP channel trafficking to modulate -cell function and insulin secretion.
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