期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 39, 页码 32346-32353出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.382499
关键词
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资金
- Medical Research Council
- Division of Signal Transduction Therapy, University of Dundee
- Cancer Research UK
- Wellcome Trust
- MRC [MC_U127070192, MC_U137761446] Funding Source: UKRI
- Cancer Research UK [12918] Funding Source: researchfish
- Medical Research Council [MC_U127070192, MC_U137761446] Funding Source: researchfish
The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. Here we describe family with sequence similarity 60A (FAM60A), a cell cycle-regulated protein that binds to the SIN3-HDAC complex. FAM60A expression peaks during G(1) and S phases of the cell cycle in U2OS cells, in a manner similar to the G(1) regulator cyclin D1, which is a known target of SIN3-HDAC. In this light we found that FAM60A binds to SIN3-HDAC-regulated promoters such as cyclin D1 in G(1) and S phases. Cells depleted of FAM60A show increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry. The data in this study introduce FAM60A as a novel regulator of SIN3-HDAC function and gene expression.
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