期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 41, 页码 34349-34360出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.410613
关键词
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资金
- National Institutes of Health [R01-DK059767]
- Colorado Nutrition and Obesity Research Center (NORC) [NIH-P30DK048520]
- UCD Diabetes and Endocrinology Research Center Pilot and Feasibility Grant [P30DK57516]
- American Heart Association [09BGIA2060705]
- American Diabetes Association
Strong evidence exists for a link between chronic low level inflammation and dietary-induced insulin resistance; however, little is known about the transcriptional networks involved. Here we show that high fat diet (HFD) or saturated fatty acid exposure directly activates CCAAT/enhancer-binding protein beta(C/EBP beta) protein expression in liver, adipocytes, and macrophages. Global C/EBP beta deletion prevented HFD-induced inflammation and surprisingly increased mitochondrial gene expression in white adipose tissue along with brown adipose tissue markers PRDM16, CIDEa, and UCP1, consistent with a resistance to HFD-induced obesity. In isolated peritoneal macrophages from C/EBP beta(-/-) mice, the anti-inflammatory gene LXR alpha and its targets SCD1 and DGAT2 were strikingly up-regulated along with IL-10, while NLRP3, a gene important for activating the inflammasome, was suppressed in response to palmitate. Using RAW 264.7 macrophage cells or 3T3-L1 adipocytes, C/EBP beta knockdown prevented palmitate-induced inflammation and p65-NF kappa B DNA binding activity, while C/EBP beta overexpression induced NF kappa B binding, JNK activation, and pro-inflammatory cytokine gene expression directly. Finally, chimeric bone marrow mice transplanted with bone marrow lacking C/EBP beta(-/-) demonstrated reduced systemic and adipose tissue inflammatory markers, macrophage content, and maintained insulin sensitivity on HFD. Taken together, these results demonstrate that HFD or palmitate exposure triggers C/EBP beta expression that controls expression of distinct aspects of alternative macrophage activation. Reducing C/EBP beta in macrophages confers protection from HFD-induced systemic inflammation and insulin resistance, suggesting it may be an attractive therapeutic target for ameliorating obesity-induced inflammatory responses.
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