期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 11, 页码 8286-8296出版社
ELSEVIER
DOI: 10.1074/jbc.M111.274274
关键词
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资金
- National Science Foundation [IOS-0818740, EPS 0236913, MCB 0455318, 0920663, DBI 0521587]
- United States Department of Agriculture [2007-35318-18393]
- United States Department of Energy [DE-SC0001295]
- Kansas Technology Enterprise Corporation
- Kansas IDeA Network of Bio-medical Research Excellence (K-INBRE)
- National Institutes of Health [P20RR16475]
- Kansas State University
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0818740] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0843312] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0920663, 0920681] Funding Source: National Science Foundation
Phosphatidic acid (PA) and phytosphingosine 1-phosphate (phyto-S1P) both are lipid messengers involved in plant response to abscisic acid (ABA). Our previous data indicate that PA binds to sphingosine kinase (SPHK) and increases its phyto-S1P-producing activity. To understand the cellular and physiological functions of the PA-SPHK interaction, we isolated Arabidopsis thaliana SPHK mutants sphk1-1 and sphk2-1 and characterized them, together with phospholipase D alpha 1 knockout, pld alpha 1, in plant response to ABA. Compared with wild-type (WT) plants, the SPHK mutants and pld alpha 1 all displayed decreased sensitivity to ABA-promoted stomatal closure. Phyto-S1P promoted stomatal closure in sphk1-1 and sphk2-1, but not in pld alpha 1, whereas PA promoted stomatal closure in sphk1-1, sphk2-1, and pld alpha 1. The ABA activation of PLD alpha 1 in leaves and protoplasts was attenuated in the SPHK mutants, and the ABA activation of SPHK was reduced in pld alpha 1. In response to ABA, the accumulation of long-chain base phosphates was decreased in pld alpha 1, whereas PA production was decreased in SPHK mutants, compared with WT. Collectively, these results indicate that SPHK and PLD alpha 1 act together in ABA response and that SPHK and phyto-S1P act upstream of PLD alpha 1 and PA in mediating the ABA response. PA is involved in the activation of SPHK, and activation of PLD alpha 1 requires SPHK activity. The data suggest that SPHK/phyto-S1P and PLD alpha 1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis.
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