期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 29, 页码 24239-24254出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.371898
关键词
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资金
- National Institutes of Health [P01 AI82362, R01 AI36082]
- International AIDS Vaccine Initiative (IAVI)
- Canadian Institutes of Health Research (CIHR)
- Vidi from the Netherlands Organization for Scientific Research (NWO)
- European Research Council [ERC-StG-2011-280829-SHEV]
- National Institutes of Health through the National Center for Research Resources' P41 program [RR017573]
The trimeric envelope glycoprotein complex (Env) is the focus of vaccine development programs aimed at generating protective humoral responses to human immunodeficiency virus type 1 (HIV-1). N-Linked glycans, which constitute almost half of the molecular mass of the external Env domains, produce considerable structural heterogeneity and are a major impediment to crystallization studies. Moreover, by shielding the peptide backbone, glycans can block attempts to generate neutralizing antibodies against a substantial subset of potential epitopes when Env proteins are used as immunogens. Here, we describe the partial deglycosylation of soluble, cleaved recombinant Env trimers by inhibition of the synthesis of complex N-glycans during Env production, followed by treatment with glycosidases under conditions that preserve Env trimer integrity. The partially deglycosylated trimers are stable, and neither abnormally sensitive to proteolytic digestion nor prone to aggregation. Moreover, the deglycosylated trimers retain or increase their ability to bind CD4 and antibodies that are directed to conformational epitopes, including the CD4-binding site and the V3 region. However, as expected, they do not react with glycan-dependent antibodies 2G12 and PGT123, or the C-type lectin receptor DC-SIGN. Electron microscopic analysis shows that partially deglycosylated trimers have a structure similar to fully glycosylated trimers, indicating that removal of glycans does not substantially perturb the structural integrity of the trimer. The gly-can-depleted Env trimers should be useful for structural and immunogenicity studies.
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