期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 52, 页码 43472-43481出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M112.428466
关键词
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资金
- Spanish Ministry of Science and Innovation [EU2008-0170]
- Fondo de Investigaciones Sanitarias [PI080771]
- Spanish Ministry of Science and Innovation
- Networking Centre on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN)
- Instituto de Salud Carlos III
Human SMC2 is part of the condensin complex, which is responsible for tightly packaging replicated genomic DNA prior to segregation into daughter cells. Engagement of the WNT signaling pathway is known to have a mitogenic effect on cells, but relatively little is known about WNT interaction with mitotic structural organizer proteins. In this work, we described the novel transcriptional regulation of SMC2 protein by direct binding of the beta-catenin.TCF4 transcription factor to the SMC2 promoter. Furthermore, we identified the precise region in the SMC2 promoter that is required for beta-catenin-mediated promoter activation. Finally, we explored the functional significance of down-regulating SMC2 protein in vivo. Treatment of WNT-activated intestinal tumor cells with SMC2 siRNA significantly reduced cell proliferation in nude mice, compared with untreated controls (p = 0.02). Therefore, we propose that WNT signaling can directly activate SMC2 transcription as a key player in the mitotic cell division machinery. Furthermore, SMC2 represents a new target for oncological therapeutic intervention.
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