期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 24, 页码 21324-21329出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.C111.245597
关键词
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资金
- National Institutes of Health [HL-033333, HL-093671, AR-44657, GM57249]
- American Heart Association [0525951T]
Heart mitochondria utilize multiple Ca2+ transport mechanisms. Among them, the mitochondrial ryanodine receptor provides a fast Ca2+ uptake pathway across the inner membrane to control excitation and metabolism coupling. In the present study, we identified a novel ryanodine-sensitive channel in the native inner membrane of heart mitochondria and characterized its pharmacological and biophysical properties by directly patch clamping mitoplasts. Four distinct channel conductances of similar to 100, similar to 225, similar to 700, and similar to 1,000 picosiemens (pS) in symmetrical 150 mM CsCl were observed. The 225 pS cation-selective channel exhibited multiple subconductance states and was blocked by high concentrations of ryanodine and ruthenium red, known inhibitors of ryanodine receptors. Ryanodine exhibited a concentration-dependent modulation of this channel, with low concentrations stabilizing a subconductance state and high concentrations abolishing activity. The 100, 700, and 1,000 pS conductances exhibited different channel characteristics and were not inhibited by ryanodine. Taken together, these findings identified a novel 225 pS channel as the native mitochondrial ryanodine receptor channel activity in heart mitoplasts with biophysical and pharmacological properties that distinguish it from previously identified mitochondrial ion channels.
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