期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 8, 页码 5492-5506出版社
ELSEVIER
DOI: 10.1074/jbc.M111.283499
关键词
-
资金
- National Institutes of Health [RO1 CA39481, RO1 CA47282, RO1 CA120975, T32 AA07463]
Decorin, a small leucine-rich proteoglycan, inhibits tumor growth by antagonizing multiple receptor tyrosine kinases including EGFR and Met. Here, we investigated decorin during normoxic angiogenic signaling. An angiogenic PCR array revealed a profound decorin-evoked transcriptional inhibition of pro-angiogenic genes, such as HIF1A. Decorin evoked a reduction of hypoxia inducible factor (HIF)-1 alpha and vascular endothelial growth factor A (VEGFA) in MDA-231 breast carcinoma cells expressing constitutively-active HIF-1 alpha. Suppression of Met with decorin or siRNA evoked a similar reduction of VEGFA by attenuating downstream beta-catenin signaling. These data establish a noncanonical role for beta-catenin in regulating VEGFA expression. We found that exogenous decorin induced expression of thrombospondin-1 and TIMP3, two powerful angiostatic agents. In contrast, decorin suppressed both the expression and enzymatic activity of matrix metalloprotease (MMP)-9 and MMP-2, two pro-angiogenic proteases. Our data establish a novel duality for decorin as a suppressor of tumor angiogenesis under normoxia by simultaneously down-regulating potent pro-angiogenic factors and inducing endogenous anti-angiogenic agents.
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