Pathway Profiling in Mycobacterium tuberculosis ELUCIDATION OF CHOLESTEROL-DERIVED CATABOLITE AND ENZYMES THAT CATALYZE ITS METABOLISM

Mycobacterium tuberculosis, the bacterium that causes tuberculosis, imports and metabolizes host cholesterol during infection. This ability is important in the chronic phase of infection. Here we investigate the role of the intracellular growth operon (igr), which has previously been identified as having a cholesterol-sensitive phenotype in vitro and which is important for intracellular growth of the mycobacteria. We have employed isotopically labeled low density lipoproteins containing either [1,7,15,22,26-C-14] cholesterol or [1,7,15,22,26-C-13] cholesterol and high resolution LC/MS as tools to profile the cholesterol-derived metabolome of an igr operon-disrupted mutant (Delta igr) of M. tuberculosis. A partially metabolized cholesterol species accumulated in the Delta igr knock-out strain that was absent in the complemented and parental wild-type strains. Structural elucidation by multidimensional H-1 and C-13 NMR spectroscopy revealed the accumulated metabolite to be methyl 1 beta-(2'-propanoate)-3a alpha-H-4 alpha-(3'-propanoic acid)-7a beta-methylhexahydro5-indanone. Heterologously expressed and purified FadE28-FadE29, an acyl-CoA dehydrogenase encoded by the igr operon, catalyzes the dehydrogenation of 2'-propanoyl-CoA ester side chains in substrates with structures analogous to the characterized metabolite. Based on the structure of the isolated metabolite, enzyme activity, and bioinformatic annotations, we assign the primary function of the igr operon to be degradation of the 2'-propanoate side chain. Therefore, the igr operon is necessary to completely metabolize the side chain of cholesterol metabolites.