Structure of Human C8 Protein Provides Mechanistic Insight into Membrane Pore Formation by Complement

C8 is one of five complement proteins that assemble on bacterial membranes to form the lethal pore-like membrane attack complex (MAC) of complement. TheMACconsists of one C5b, C6, C7, and C8 and 12-18 molecules of C9. C8 is composed of three genetically distinct subunits, C8 alpha, C8 beta, and C8 gamma. The C6, C7, C8 alpha, C8 beta, and C9 proteins are homologous and together comprise the MAC family of proteins. All contain N- and C-terminal modules and a central 40-kDa membrane attack complex perforin (MACPF) domain that has a key role in forming the MACpore. Here, we report the 2.5A resolution crystal structure of human C8 purified from blood. This is the first structure of a MAC family member and of a human MACPF-containing protein. The structure shows the modules in C8 alpha and C8 beta are located on the periphery of C8 and not likely to interact with the target membrane. The C8 gamma subunit, a member of the lipocalin family of proteins that bind and transport small lipophilic molecules, shows no occupancy of its putative ligand-binding site. C8 alpha and C8 beta are related by a rotation of similar to 22 degrees with only a small translational component along the rotation axis. Evolutionary arguments suggest the geometry of binding between these two subunits is similar to the arrangement of C9 molecules within the MAC pore. This leads to a model of the MAC that explains how C8-C9 and C9-C9 interactions could facilitate refolding and insertion of putative MACPF transmembrane beta-hairpins to form a circular pore.