期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 38, 页码 33511-33519出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M111.270553
关键词
-
资金
- National Institutes of Health CIPRA [U19 AI51915]
- National Science and Technology Major [2008ZX10001-011, 2009ZX10004-016]
- National Basic Research Program (973 Program) [2006CB504200]
- National Outstanding Youth Award [30825035]
- Tsinghua University Initiative Scientific Research
- Tsinghua Yue-Yuen Medical Sciences Fund
Host antibody response is a crucial defense against pathogenic infection. Here, we report a novel technique allowing quantitative measurement of polyclonal antibody response in vivo. This involves expression of a combinatorial library of target proteins from a candidate pathogen on the surface of yeast Saccharomyces cerevisiae. After mixing with serum/plasma from infected or immunized subjects, positive yeast clones were isolated via fluorescence-activated cell sorting (FACS). Using this technique, we have studied mouse immunized serum with recombinant hemagglutinin (HA) protein from a human influenza H5N1 strain (A/Anhui/1/2005) and convalescent plasma from an infected human in China. Our technique has identified novel antigenic domains targeted by serum/plasma and allowed calculation of the relative proportion of the antibody response against each domain. We believe such systematic measurement of an antibody response is unprecedented, and applying this method to different pathogens will improve understanding of protective immunity and guide development of vaccines and therapeutics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据