Biochemical and Molecular Characterization of Secreted α-Xylosidase from Aspergillus niger

Background: Microbial secreted alpha-xylosidases are rare. Results: An alpha-xylosidase of Aspergillus niger (AxlA) was purified and expressed in Pichia pastoris. Conclusion: Native and recombinant AxlA act on pNP alpha X and isoprimeverose. Together with beta-glucosidase, AxlA degrades xyloglucan (XG) heptasaccharide. Together with xyloglucanase, beta-galactosidase, and beta-glucosidase, it degrades tamarind XG. Significance: The biochemical function of AxlA is established.