期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 287, 期 4, 页码 2632-2642出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M111.323709
关键词
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资金
- National Institutes of Health [CA109182, ES017146]
- Ministry of Science and Innovation of Spain [FIS-PI070071, SAF-2010-20198-C02-01]
- Comunidad de Madrid/Universidad Complutense de Madrid [920384]
- Samuel Waxman Cancer Research Foundation
- New York Stem Cell Science
- Comunidad de Madrid
- Ministry of Education, Spain
We reveal a novel pro-survival role for mammalian p38 alpha in response to H2O2, which involves an up-regulation of antioxidant defenses. The presence of p38 alpha increases basal and H2O2-induced expression of the antioxidant enzymes: superoxide-dismutase 1 (SOD-1), SOD-2, and catalase through different mechanisms, which protects from reactive oxygen species (ROS) accumulation and prevents cell death. p38 alpha was found to regulate (i) H2O2-induced SOD-2 expression through a direct regulation of transcription mediated by activating transcription factor 2 (ATF-2) and (ii) H2O2-induced catalase expression through regulation of protein stability and mRNA expression and/or stabilization. As a consequence, SOD and catalase activities are higher in WT MEFs. We also found that this p38 alpha-dependent antioxidant response allows WT cells to maintain an efficient activation of the mTOR/p70S6K pathway. Accordingly, the loss of p38 alpha leads to ROS accumulation in response to H2O2, which causes cell death and inactivation of mTOR/p70S6K signaling. This can be rescued by either p38 alpha re-expression or treatment with the antioxidants, N-acetyl cysteine, or exogenously added catalase. Therefore, our results reveal a novel homeostatic role for p38 alpha in response to oxidative stress, where ROS removal is favored by antioxidant enzymes up-regulation, allowing cell survival and mTOR/p70S6K activation.
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