期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 43, 页码 37181-37186出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.C111.276972
关键词
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资金
- Deutsche Forschungsgemeinschaft [SFB596]
- Bundesministerium fur Bildung und Forschung (KNDD)
- Center for Integrated Protein Science Munich (CIPSM)
- Elitenetzwerk Bayern
- LMU
gamma-Secretase is essential for the generation of the neurotoxic 42-amino acid amyloid beta-peptide (A beta(42)). The aggregation-prone hydrophobic peptide, which is deposited in Alzheimer disease (AD) patient brain, is generated from a C-terminal fragment of the beta-amyloid precursor protein by an intramembrane cleavage of gamma-secretase. Because A beta(42) is widely believed to trigger AD pathogenesis, gamma-secretase is a key AD drug target. Unlike inhibitors of the enzyme, gamma-secretase modulators (GSMs) selectively lower A beta(42) without interfering with the physiological function of gamma-secretase. The molecular target(s) of GSMs and hence the mechanism of GSM action are not established. Here we demonstrate by using a biotinylated photocross-linkable derivative of highly potent novel second generation GSMs that gamma-secretase is a direct target of GSMs. The GSM photoprobe specifically bound to the N-terminal fragment of presenilin, the catalytic subunit of gamma-secretase, but not to other gamma-secretase subunits. Binding was differentially competed by GSMs of diverse structural classes, indicating the existence of overlapping/multiple GSM binding sites or allosteric alteration of the photoprobe binding site. The beta-amyloid precursor protein C-terminal fragment previously implicated as the GSM binding site was not targeted by the compound. The identification of presenilin as the molecular target of GSMs directly establishes allosteric modulation of enzyme activity as a mechanism of GSM action and may contribute to the development of therapeutically active GSMs for the treatment of AD.
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