期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 285, 期 46, 页码 35293-35302出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.160069
关键词
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资金
- National Institutes of Health [R01CA108509]
- Ruth L. Kirschstein NRSA [F31CA142216]
TGF-beta promotes cell migration and invasion, an attribute that is linked to the pro-metastasis function of this cytokine in late stage cancers. The LIM 1863 colon carcinoma organoid undergoes epithelial-mesenchymal transition (EMT) in response to TGF-beta. This process is markedly accelerated by TNF-alpha, and we found that the levels of miR-21 and miR-31 were prominently elevated under the synergistic actions of TGF-beta/TNF-alpha. Consistent with this, overexpression of either miR-21 or miR-31 significantly enhanced the effect of TGF-beta alone on LIM 1863 morphological changes. More importantly, transwell assays demonstrated the positive effects of both miR-21 and miR-31 in TGF-beta regulation of LIM 1863 motility and invasiveness. Elevated levels of miR-21 and miR-31 also enhanced motility and invasiveness of other colon carcinoma cell lines. We present compelling evidence that TIAM1, a guanidine exchange factor of the Rac GTPase, is a direct target of both miR-21 and miR-31. Indeed in LIM 1863 cells, suppression of TIAM1 is required for miR-21/miR-31 to enhance cell migration and invasion. Therefore, we have uncovered miR-21 and miR-31 as downstream effectors of TGF-beta in facilitating invasion and metastasis of colon carcinoma cells.
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