期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 285, 期 18, 页码 13781-13787出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.107300
关键词
-
资金
- National Institutes of Health [2R01AR43140, 5P01 AR052354]
The control of resting free Ca2+ in skeletal muscle is thought to be a balance of channels, pumps, and exchangers in both the sarcolemma and sarcoplasmic reticulum. We explored these mechanisms using pharmacologic and molecular perturbations of genetically engineered (dyspedic) muscle cells that constitutively lack expression of the skeletal muscle sarcoplasmic reticulum Ca2+ release channels, RyR1 and RyR3. We demonstrate here that expression of RyR1 is responsible for more than half of total resting Ca2+ concentration ([Ca2+](rest)) measured in wild type cells. The elevated [Ca2+](rest) in RyR1-expressing cells is not a result of active gating of the RyR1 channel but instead is accounted for by the RyR1 ryanodine-insensitive Ca2+ leak conformation. In addition, we demonstrate that basal sarcolemmal Ca2+ influx is also governed by RyR1 expression and contributes in the regulation of [Ca2+](rest) in skeletal myotubes.
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