期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 286, 期 4, 页码 2486-2491出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M110.190843
关键词
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资金
- National Institutes of Health [GM089778]
- Ministerio de Ciencia e Innovacion [BFU2008-03778]
- UCLA Jonsson Cancer Center
- Ministerio de Educacion y Ciencia
- Generalitat de Catalunya
An intra-S phase checkpoint slows the rate of DNA replication in response to DNA damage and replication fork blocks in eukaryotic cells. In the budding yeast Saccharomyces cerevisiae, such down-regulation is achieved through the Rad53 kinase-dependent block of origins of replication. We have identified the Rad53 phosphorylation sites on Dbf4, the activator subunit of the essential S phase Dbf4-dependent kinase, and generated a non-phosphorylatable Dbf4 mutant (dbf4(7A)). We show here that dbf4(7A) is a bona fide intra-S phase checkpoint bypass allele that contributes to abrogating the Rad53 block of origin firing in response to genotoxic stress.
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