期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 285, 期 45, 页码 34677-34685出版社
ELSEVIER
DOI: 10.1074/jbc.M110.164350
关键词
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资金
- Deutsche Forschungsgemeinschaft [SCHI 1012/1-1]
- Netherlands Organisation for Scientific Research (NWO) [916-66-019, 050-10-014]
- EU (European Vascular Genomics Network) [LSHM-CT-2003-503254]
Increased interferon (IFN)-beta signaling in patients with insufficient coronary collateralization and an inhibitory effect of IFN beta on collateral artery growth in mice have been reported. The mechanisms of IFN beta-induced inhibition of arteriogenesis are unknown. In stimulated monocytes from patients with chronic total coronary artery occlusion and decreased arteriogenic response, whole genome expression analysis showed increased expression of IFN beta-regulated genes. Immunohistochemically, the IFN beta receptor was localized in the vascular media of murine collateral arteries. Treatment of vascular smooth muscle cells (VSMC) with IFN beta resulted in an attenuated proliferation, cell-cycle arrest, and increased expression of cyclin-dependent kinase inhibitor-1A (p21). The growth inhibitory effect of IFN beta was attenuated by inhibition of p21 by RNA interference. IFN beta-treated THP1 monocytes showed enhanced apoptosis. Subsequently, we tested if collateral artery growth can be stimulated by inhibition of IFN beta-signaling. RNA interference of the IFN beta receptor-1 (IFNAR1) increased VSMC proliferation, cell cycle progression, and reduced p21 gene expression. IFN beta signaling and FAS and TRAIL expression were attenuated in monocytes from IFNAR1(-/-) mice, indicating reduced monocyte apoptosis. Hindlimb perfusion restoration 1 week after femoral artery ligation was improved in IFNAR1(-/-) mice compared with wild-type mice as assessed by infusion of fluorescent microspheres. These results demonstrate that IFN beta inhibits collateral artery growth and VSMC proliferation through p21-dependent cell cycle arrest and induction of monocyte apoptosis. Inhibition of IFN beta stimulates VSMC proliferation and collateral artery growth.
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