期刊
ACTA BIOMATERIALIA
卷 16, 期 -, 页码 196-205出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.01.002
关键词
HB5 aptamer; Mesoporous silica-carbon nanoparticles; Chemo-photothermal therapy; HER2-positive; Breast cancer
资金
- National Key Basic Research Program of China (973 Program) [2013CB932502]
- National Natural Science Foundation of China [21303022]
- Zhuo Xue Talent Plan of Fudan University
- Natural Science Foundation of Shanghai City of China [13ZR1451400]
- Sino-German Research Project [GZ995]
Tumor-specific therapeutic platforms designed for combined tumor therapy has recently received wide attention. In this work, a new HB5 aptamer-functionalized mesoporous silica-carbon based doxorubicin (DOX)-loaded system (MSCN-PEG-HB5/DOX) was successfully constructed and characterized for chemophotothermal combined therapy of human epithelial growth factor receptor 2 (HER2)-positive breast cancer cells. The in vitro release result showed that MSCN-PEG-HB5/DOX exhibited pH-sensitive and NIR-triggered release manner. HB5-modified nanoparticles showed significant higher cellular uptake in HER2-positive breast cancer cells (SK-BR-3) but not in normal breast epithelial cells (MCF-10A), compared to unmodified counterparts. The intracellular uptake of functional nanoparticles was mainly based on the receptor-mediated mechanism which was energy-dependent. Cytotoxicity experiments demonstrated that combined therapy induced highest cell killing effect compared to chemotherapy and photothermal therapy alone. The combination index (Cl) was 0.253 indicating the synergistic effect of chemotherapy and photothermal therapy. These findings suggested that MSCN-PEG-HB5/DOX was a potential chemo-photothermal therapeutic platform targeting to HER2-positive breast cancers. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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