期刊
ACTA BIOMATERIALIA
卷 18, 期 -, 页码 155-167出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2015.02.023
关键词
Antimicrobial peptide; Amphipathicity; Cell selectivity; Helix; Mechanism
资金
- National Natural Science Foundation of China [31472104, 31272453]
- National Key Scientific and Technological Project [2013BAD10B03]
- China Agriculture Research System [CARS-36]
- Program for Innovative Research Team of Universities in the Heilongjiang Province [2012TD003]
Antimicrobial peptides (AMPS) serve as a defense mechanism within multicellular organisms and are attracting increasing attention because of their potential application in the treatment of multidrug-resistant infections. Amphipathicity has long been believed to be the most important consideration for the structural modification and design of both naturally occurring and synthetic AMPs. Previous studies indicated that disruptive amphipathicity formed by replacing the paired charged amino acid residues on the polar face of an amphipathic helix with tryptophan residues linked with hydrogen bonds on the basis of alpha-helical protein folding principles endowed the AMPs with increased cell selectivity. In an attempt to augment and hone this strategy further, we designed a series of imperfect amphipathic peptides by placing different types of amino acid residues at the hydrogen bond linked positions of alpha-helix structures to characterize their antimicrobial properties and mechanism of action. The D-Trp-substituted sequence (PRW4-d) showed greater antimicrobial potency than Cys-(C4), Asp-(D4), Ile-(I4), and Pro-(P4) substituted sequences, comparable to the L-Trp-substituted parent sequence (PRW4). Furthermore, the total replacement of Lys residues with Arg residues along the peptide sequence (PRW4-R) exhibited enhanced antimicrobial activity and cell selectivity. In addition, no cytotoxicity was observed among these synthetic peptides. PRW4-d and PRW4-R maintained their activities in the presence of physiological salts and human serum. The fluorescence spectroscopy, flow cytometry, and electron microscopy observations indicated that the optimized sequences exhibited excellent antimicrobial potency by inducing cytoplasmic membrane potential loss, membrane permeabilization and disruption. Collectively, the results could be useful for designing short AMPs with great antimicrobial activity and cell selectivity. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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