Compartmentalization of Phosphatidylinositol 4,5-Bisphosphate Signaling Evidenced Using Targeted Phosphatases

Phosphatidylinositol 4,5-bisphosphate (PIP2) is a prevalent phosphoinositide in cell membranes, with important functions in cell signaling and activation. A large fraction of PIP2 associates with the detergent-resistant membrane raft fraction, but the functional significance of this association remains controversial. To measure the properties of raft and nonraft PIP2 in cell signaling, we targeted the PIP2-specific phosphatase Inp54p to either the raft or nonraft membrane fraction using minimal membrane anchors. Interestingly, we observed that targeting Inp54p to the nonraft fraction resulted in an enrichment of raft-associated PIP2 and striking changes in cell morphology, including a wortmannin-sensitive increase in cell filopodia and cell spreading. In contrast, raft-targeted Inp54p depleted the raft pool of PIP2 and produced smooth T cells void of membrane ruffling and filopodia. Furthermore, raft-targeted Inp54p inhibited capping in T cells stimulated by cross-linking the T cell receptor, but without affecting the T cell receptor-dependent Ca2+ flux. Altogether, these results provide evidence of compartmentalization of PIP2-dependent signaling in cell membranes such as predicted by the membrane raft model.