期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 283, 期 39, 页码 26423-26427出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M803237200
关键词
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资金
- Deutsche Forschungsgemeinschaft, the Sonderforschungsbereich [SFB646]
- Nanoscience Initiative Munich NIM, the Elitenetzwerk Bayern
- Transregio 5 Chromatin
- Chemischen Industrie
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
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