期刊
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
卷 43, 期 2, 页码 149-162出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10863-011-9341-z
关键词
Tl+; H+; Li+; Na+; K+; Ca2+; Mitochondrial permeability transition; Complex I; Adenine nucleotide translocase; Mitochondrial respiration; Mitochondrial swelling; Membrane potential
资金
- Magnus Ehrnrooth Foundation
The Tl+-induced opening of the MPTP in Ca2+-loaded rat liver mitochondria energized by respiration on the substrates succinate or glutamate plus malate was recorded as increased swelling and dissipation of mitochondrial membrane potential as well as decreased state 4, or state 3, or 2,4-dinitrophenol-stimulated respiration. These effects of Tl+ increased in nitrate media containing monovalent cations in the order of Li+ < NH (4) (+) a parts per thousand currency sign Na+ < K+. They were potentiated by inorganic phosphate and diminished by the MPTP inhibitors (ADP, CsA, Mg2+, Li+, rotenone, EGTA, and ruthenium red) both individually and more potently in their combinations. Maximal swelling of both non-energized and energized Ca2+-loaded mitochondria in rotenone-free media is an indication of Ca2+ uptake driven by respiration on mitochondrial endogenous substrates. It is suggested that Tl+ (distinct from Cd2+, Hg2+, and other heavy metals and regardless of the used respiratory substrates) can stimulate opening of the MPTP only in the presence of Ca2+. We discuss the possible participation of Ca2+-binding sites, located near the respiratory complex I and the adenine nucleotide translocase, in inducing opening of the MPTP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据