期刊
JOURNAL OF BIOCHEMISTRY
卷 150, 期 5, 页码 545-552出版社
OXFORD UNIV PRESS
DOI: 10.1093/jb/mvr094
关键词
Agrocybe cylindracea galectin; frontal affinity chromatography; hydrogen bond; saturation mutagenesis; sialo-binding lectin
资金
- Kanto Bureau of Economy, Trade and Industry in Japan
- Grants-in-Aid for Scientific Research [21370062] Funding Source: KAKEN
Sialic acid represents a critical sugar component located at the outermost position of glycoconjugates, playing important roles in extensive biological processes. To date, however, there have been only few probes which show affinity to alpha(2,3)-linked sialic acid-containing glycoconjugates. Agrocybe cylindracea galectin is known to have a relatively high affinity towards Neu5Ac alpha(2,3)Gal beta(1,4)Glc (3'-sialyl lactose), but it significantly recognizes various beta-galactosides, such as Gal beta(1,4)GlcNAc beta (LacNAc) and Gal beta(1,3)GalNAc alpha (T-antigen). To eliminate this background specificity, we focused an acidic amino acid residue (Glu86), which interacts with the glucose unit of 3'-sialyl lactose and substituted it with all other amino acids. Carbohydrate-binding specificity of the derived 14 mutants was analysed by surface plasmon resonance, and it was found that E86D mutant (Glu86 substituted with Asp) substantially lost the binding ability to LacNAc and T-antigen, while it retained the high affinity for 3'-sialyl lactose. Further, frontal affinity chromatography analysis using 132 pyridylaminated oligosaccharides confirmed that the E86D mutant had a strong preference for alpha(2,3)-disialo biantennary N-linked glycan. However, it showed the large decrease in the affinity for any of the asialo complex-type N-glycans and the glycolipid-type glycans. Thus, the developed mutant E86D will be of practical use in various fields relevant to cell biology and glycotechnology.
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